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gripna myd88  (Active Motif)


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    Structured Review

    Active Motif gripna myd88
    EDN-induced IL-6 production was dependent on <t>MyD88.</t> (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and <t>MyD88</t> <t>knockout</t> (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.
    Gripna Myd88, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gripna+myd88/anti+myd88/pmc02234357-149-14-17
    Average 90 stars, based on 1 article reviews
    gripna myd88 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses"

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20062027

    EDN-induced IL-6 production was dependent on MyD88. (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and MyD88 knockout (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.
    Figure Legend Snippet: EDN-induced IL-6 production was dependent on MyD88. (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and MyD88 knockout (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.

    Techniques Used: Derivative Assay, Transfection, Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Generated, Knock-Out

    Related Articles

    Derivative Assay:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.

    Transfection:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.

    Incubation:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.

    Concentration Assay:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.

    Generated:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.

    Knock-Out:

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses
    Article Snippet: Subsequently, iDCs were incubated in fresh G4 medium in the absence or presence of EDN, Pam3 ( N -palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cys-[S]-serl-[S]-lys [reference ] trihydrochloride; InvivoGen), FSL-1 (InvivoGen), or LPS ( E. coli O55:B5; Sigma-Aldrich) at specified concentrations for 24∼48 h in a CO 2 (5%) incubator before analyzing their phenotype and function.



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    gripna myd88  (Active Motif)
    90
    Active Motif gripna myd88
    EDN-induced IL-6 production was dependent on <t>MyD88.</t> (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and <t>MyD88</t> <t>knockout</t> (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.
    Gripna Myd88, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gripna+myd88/anti+myd88/pmc02234357-149-14-17
    Average 90 stars, based on 1 article reviews
    gripna myd88 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    EDN-induced IL-6 production was dependent on MyD88. (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and MyD88 knockout (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.

    Journal: The Journal of Experimental Medicine

    Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses

    doi: 10.1084/jem.20062027

    Figure Lengend Snippet: EDN-induced IL-6 production was dependent on MyD88. (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and MyD88 knockout (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.

    Article Snippet: For the transient silencing of DC MyD88, monocyte-derived iDCs were transduced without or with gripNA MyD88 (5′-CCTGCAGCCAYDDCGGGC-3′; Active Motif) at 10 nmol/10 6 cells using Chariot II (Active Motif) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Transfection, Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Generated, Knock-Out